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resource source identifier monarch genomic dna purification kit neb  (New England Biolabs)


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    New England Biolabs resource source identifier monarch genomic dna purification kit neb
    Resource Source Identifier Monarch Genomic Dna Purification Kit Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1049 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+source+identifier+monarch+genomic+dna+purification+kit+neb/Monarch+Spin+gDNA+Extraction+Kit/pm36657393-575-2-10
    Average 99 stars, based on 1049 article reviews
    resource source identifier monarch genomic dna purification kit neb - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    DNA Purification:

    Article Title: Gene-by-gene screen of the unknown proteins encoded on Plasmodium falciparum chromosome 3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies monoclonal mouse anti-GFP Roche Cat# 11814460001; RRID: AB_390913 mouse anti-FKBP12 Santa Cruz Cat# SC-136962; RRID: AB_2102734 rabbit anti-Aldolase Mesén-Ramı́rez et al.45 N/A HRP conjugated goat anti-mouse Dianova Cat# DAB-87613 HRP conjugated anti-rabbit Dianova Cat# 111-035-045; RRID: AB_2337938 HRP conjugated Streptavidin Thermo Fisher Scientific Cat# SA10001; RRID: AB_2534069 Alexa FluorTM 594 conjugated Streptavidin Thermo Fisher Scientific Cat# S32356 Alexa FluorTM 488 conjugated goat anti-mouse Thermo Fisher Scientific Cat# A-11001 Bacterial and virus strains Escherichia coli XL-10 gold Tetr D(mcrA)183 D(mcrCBhsdSMRmrr) 173 endA1 supE44 thi-1recA1 gyrA96 relA1 lac Hte [F’ proABlacIqZ DM15 Tn10 (Tetr) Amy Camr] New England Biolabs Cat# C3040 Biological samples 0+ red blood cells (transfusion blood) University Medical Center Hamburg-Eppendorf (UKE) N/A Chemicals, peptides, and recombinant proteins WR99210 Jacobus Pharmaceuticals N/A Blasticidin S (BSD) Life Technologies Cat# R21001 DSM1 (MRA-1161) MRA/BEI resources Cat# MRA 1161 G418 Sigma Aldrich Cat# A1720 Rapalog (A/C Heterodimerizer AP21967) Clontech Cat# 635055 DAPI (4’,6’-diamidine-2’-phenylindole dihydrochloride) Roche Cat# 10236276001 Tubulin TrackerTM Deep Red Thermo Fisher Scientific Cat# T34077 Mito Tracker RedTM Thermo Fisher Scientific Cat# M22425 Hoechst 33342 Cayman Cat# K9061 Dihydroethidium Cayman Cat# 12013 Percoll GE Healthcare Cat# 17-0891-01 Phusion High-Fidelity DNA-Polymerase New England Biolabs Cat# D22914 KAPA HiFi HotStart Roche Cat# KK2502 FirePol Solis BioDyne Cat# 01-01-02000 Biotin Sigma Aldrich Cat# B4639-100MG Streptavidin Sepharose GE Healthcare Cat# 17-5113-01 Triethylammonium bicarbonate buffer (TEAB) Sigma Aldrich Cat# T7408-500ML Tris(2-carboethyl)phosphine hydrochloride (TCEP) Sigma Aldrich Cat# C4706-2G Methyl methanethiosulfonate (MMTS) Thermo Fisher Scientific Cat# 23011 Iodoacetamide (IAA) Sigma Aldrich Cat# I1149-25G Trypsin/LysC Promega Cat# V5072 37% formaldehyde solution Sigma Aldrich Cat# 252549-25ML 20% formaldehyde-13C d2 solution Sigma Aldrich Cat# 596388-20G NaBH3CN Merck Cat# 818053 NaBD3CN Sigma Aldrich Cat# 190020-1G Critical commercial assays QIAGEN Plasmid Midi Kit Qiagen Cat# 12143 (Continued on next page) e1 Cell Systems 14, 9–23.e1–e7, January 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Monarch Genomic DNA Purification Kit NEB Cat# T3010S Deposited data Mass spectrometry data This study PRIDE: PXD035332 Experimental models: Cell lines Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-terminally fused to 2xFKBP-GFP-2xFKBP (C-termintegration cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) Wichers et al.22 N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-terminally fused to 2xFKBP-GFP2xFKBP (N-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-terminally fused to 2xFKBP-GFP2xFKBP (N-term-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-termintegration episomally expressing lyn-FRBmCherry or 1xNLS-FRB-mCherry (KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) episomally expressing 1xNLS-FRBmCherry (KS cell line) Wichers et al. 22 N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C3, ChrC13, Chr3C18 C-term-integration episomally expressing marker-mCherry (Colocalisation cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C7, ChrC16, SICs and NDC80 C-termintegration episomally expressing markermCherry and lyn-FRB (Colocalisation and KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-CL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C16 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Experimental models: Organisms/strains Plasmodium falciparum 3D7 Walliker et al.46 N/A (Continued on next page) Cell Systems 14, 9–23.e1–e7, January 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides For oligonucleotides used in this study see Table S2 this study N/A Recombinant DNA pSLI-sandwich Birnbaum et al.19 N/A pSLI- Chr3C1-11, Chr313-33, SIC1-5, NDC80-sandwich (see Table S2) this study N/A Chr3C12 pSLI-sandwich Wichers et al.22 N/A p-N-SLI-sandwich-K13-loxP Birnbaum et al.19 N/A p-N-SLI-Chr3C30-sandwich-loxP this study N/A p-N-SLI-Chr3C32-sandwich-loxP this study N/A 1xNLS-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A lyn-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A pSkipFlox Birnbaum et al.19 N/A pLyn-FRB-T2A-mCherry-yDHODHnmd3 Hoeijmakers et al.40 N/A pGRASP-mCherry-BSDnmd3 Birnbaum et al.20 N/A pARL-ama1ARO-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1RON12-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1AMA1-mCherry-yDHODH Wichers et al.22 N/A CEN3-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A NDC80-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A SKA-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A Chr3C16-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A BirA*-NL Birnbaum et al.20 N/A BirA*-CL Birnbaum et al.20 N/A Software and algorithms AxioVision release 4.7 Zeiss www.micro-shop.zeiss.com/en/ us/system/axiovision+softwaresoftware+axiovision-software/6014/ CorelDRAW X6 Corel Corporation www.coreldraw.com CorelPHOTOPAINT X6 Corel Corporation www.coreldraw.com FACSDiva version 6.1.3 BD Biosciences www.bdbiosciences.com/en-us/ products/software/instrumentsoftware/bd-facsdiva-software Graph Pad Prism 9 Graph Pad Software www.graphpad.com/scientific- software/prism/ ImageJ 2 open source47 https://imagej.nih.gov/ij/ ImageLab v5.2.1 Bio-Rad Laboratories www.bio-rad.com/de-de/ product/image-lab-software PyMOL Molecular Graphics System 2.5.2 Schrödinger, LLC https://pymol.org/2/ MaxQuant 1.5.3.30 open source from Cox lab (Cox et al.48) https://maxquant.org/ MaxQuant Perseus software package 1.4.0.20 open source from Cox lab (Tyanova et al.49) https://maxquant.org/perseus/ R i386 3.3.3 Open source https://www.r-project.org/

    Mass Spectrometry:

    Article Title: Gene-by-gene screen of the unknown proteins encoded on Plasmodium falciparum chromosome 3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies monoclonal mouse anti-GFP Roche Cat# 11814460001; RRID: AB_390913 mouse anti-FKBP12 Santa Cruz Cat# SC-136962; RRID: AB_2102734 rabbit anti-Aldolase Mesén-Ramı́rez et al.45 N/A HRP conjugated goat anti-mouse Dianova Cat# DAB-87613 HRP conjugated anti-rabbit Dianova Cat# 111-035-045; RRID: AB_2337938 HRP conjugated Streptavidin Thermo Fisher Scientific Cat# SA10001; RRID: AB_2534069 Alexa FluorTM 594 conjugated Streptavidin Thermo Fisher Scientific Cat# S32356 Alexa FluorTM 488 conjugated goat anti-mouse Thermo Fisher Scientific Cat# A-11001 Bacterial and virus strains Escherichia coli XL-10 gold Tetr D(mcrA)183 D(mcrCBhsdSMRmrr) 173 endA1 supE44 thi-1recA1 gyrA96 relA1 lac Hte [F’ proABlacIqZ DM15 Tn10 (Tetr) Amy Camr] New England Biolabs Cat# C3040 Biological samples 0+ red blood cells (transfusion blood) University Medical Center Hamburg-Eppendorf (UKE) N/A Chemicals, peptides, and recombinant proteins WR99210 Jacobus Pharmaceuticals N/A Blasticidin S (BSD) Life Technologies Cat# R21001 DSM1 (MRA-1161) MRA/BEI resources Cat# MRA 1161 G418 Sigma Aldrich Cat# A1720 Rapalog (A/C Heterodimerizer AP21967) Clontech Cat# 635055 DAPI (4’,6’-diamidine-2’-phenylindole dihydrochloride) Roche Cat# 10236276001 Tubulin TrackerTM Deep Red Thermo Fisher Scientific Cat# T34077 Mito Tracker RedTM Thermo Fisher Scientific Cat# M22425 Hoechst 33342 Cayman Cat# K9061 Dihydroethidium Cayman Cat# 12013 Percoll GE Healthcare Cat# 17-0891-01 Phusion High-Fidelity DNA-Polymerase New England Biolabs Cat# D22914 KAPA HiFi HotStart Roche Cat# KK2502 FirePol Solis BioDyne Cat# 01-01-02000 Biotin Sigma Aldrich Cat# B4639-100MG Streptavidin Sepharose GE Healthcare Cat# 17-5113-01 Triethylammonium bicarbonate buffer (TEAB) Sigma Aldrich Cat# T7408-500ML Tris(2-carboethyl)phosphine hydrochloride (TCEP) Sigma Aldrich Cat# C4706-2G Methyl methanethiosulfonate (MMTS) Thermo Fisher Scientific Cat# 23011 Iodoacetamide (IAA) Sigma Aldrich Cat# I1149-25G Trypsin/LysC Promega Cat# V5072 37% formaldehyde solution Sigma Aldrich Cat# 252549-25ML 20% formaldehyde-13C d2 solution Sigma Aldrich Cat# 596388-20G NaBH3CN Merck Cat# 818053 NaBD3CN Sigma Aldrich Cat# 190020-1G Critical commercial assays QIAGEN Plasmid Midi Kit Qiagen Cat# 12143 (Continued on next page) e1 Cell Systems 14, 9–23.e1–e7, January 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Monarch Genomic DNA Purification Kit NEB Cat# T3010S Deposited data Mass spectrometry data This study PRIDE: PXD035332 Experimental models: Cell lines Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-terminally fused to 2xFKBP-GFP-2xFKBP (C-termintegration cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) Wichers et al.22 N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-terminally fused to 2xFKBP-GFP2xFKBP (N-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-terminally fused to 2xFKBP-GFP2xFKBP (N-term-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-termintegration episomally expressing lyn-FRBmCherry or 1xNLS-FRB-mCherry (KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) episomally expressing 1xNLS-FRBmCherry (KS cell line) Wichers et al. 22 N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C3, ChrC13, Chr3C18 C-term-integration episomally expressing marker-mCherry (Colocalisation cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C7, ChrC16, SICs and NDC80 C-termintegration episomally expressing markermCherry and lyn-FRB (Colocalisation and KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-CL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C16 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Experimental models: Organisms/strains Plasmodium falciparum 3D7 Walliker et al.46 N/A (Continued on next page) Cell Systems 14, 9–23.e1–e7, January 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides For oligonucleotides used in this study see Table S2 this study N/A Recombinant DNA pSLI-sandwich Birnbaum et al.19 N/A pSLI- Chr3C1-11, Chr313-33, SIC1-5, NDC80-sandwich (see Table S2) this study N/A Chr3C12 pSLI-sandwich Wichers et al.22 N/A p-N-SLI-sandwich-K13-loxP Birnbaum et al.19 N/A p-N-SLI-Chr3C30-sandwich-loxP this study N/A p-N-SLI-Chr3C32-sandwich-loxP this study N/A 1xNLS-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A lyn-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A pSkipFlox Birnbaum et al.19 N/A pLyn-FRB-T2A-mCherry-yDHODHnmd3 Hoeijmakers et al.40 N/A pGRASP-mCherry-BSDnmd3 Birnbaum et al.20 N/A pARL-ama1ARO-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1RON12-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1AMA1-mCherry-yDHODH Wichers et al.22 N/A CEN3-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A NDC80-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A SKA-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A Chr3C16-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A BirA*-NL Birnbaum et al.20 N/A BirA*-CL Birnbaum et al.20 N/A Software and algorithms AxioVision release 4.7 Zeiss www.micro-shop.zeiss.com/en/ us/system/axiovision+softwaresoftware+axiovision-software/6014/ CorelDRAW X6 Corel Corporation www.coreldraw.com CorelPHOTOPAINT X6 Corel Corporation www.coreldraw.com FACSDiva version 6.1.3 BD Biosciences www.bdbiosciences.com/en-us/ products/software/instrumentsoftware/bd-facsdiva-software Graph Pad Prism 9 Graph Pad Software www.graphpad.com/scientific- software/prism/ ImageJ 2 open source47 https://imagej.nih.gov/ij/ ImageLab v5.2.1 Bio-Rad Laboratories www.bio-rad.com/de-de/ product/image-lab-software PyMOL Molecular Graphics System 2.5.2 Schrödinger, LLC https://pymol.org/2/ MaxQuant 1.5.3.30 open source from Cox lab (Cox et al.48) https://maxquant.org/ MaxQuant Perseus software package 1.4.0.20 open source from Cox lab (Tyanova et al.49) https://maxquant.org/perseus/ R i386 3.3.3 Open source https://www.r-project.org/

    Knock-In:

    Article Title: Gene-by-gene screen of the unknown proteins encoded on Plasmodium falciparum chromosome 3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies monoclonal mouse anti-GFP Roche Cat# 11814460001; RRID: AB_390913 mouse anti-FKBP12 Santa Cruz Cat# SC-136962; RRID: AB_2102734 rabbit anti-Aldolase Mesén-Ramı́rez et al.45 N/A HRP conjugated goat anti-mouse Dianova Cat# DAB-87613 HRP conjugated anti-rabbit Dianova Cat# 111-035-045; RRID: AB_2337938 HRP conjugated Streptavidin Thermo Fisher Scientific Cat# SA10001; RRID: AB_2534069 Alexa FluorTM 594 conjugated Streptavidin Thermo Fisher Scientific Cat# S32356 Alexa FluorTM 488 conjugated goat anti-mouse Thermo Fisher Scientific Cat# A-11001 Bacterial and virus strains Escherichia coli XL-10 gold Tetr D(mcrA)183 D(mcrCBhsdSMRmrr) 173 endA1 supE44 thi-1recA1 gyrA96 relA1 lac Hte [F’ proABlacIqZ DM15 Tn10 (Tetr) Amy Camr] New England Biolabs Cat# C3040 Biological samples 0+ red blood cells (transfusion blood) University Medical Center Hamburg-Eppendorf (UKE) N/A Chemicals, peptides, and recombinant proteins WR99210 Jacobus Pharmaceuticals N/A Blasticidin S (BSD) Life Technologies Cat# R21001 DSM1 (MRA-1161) MRA/BEI resources Cat# MRA 1161 G418 Sigma Aldrich Cat# A1720 Rapalog (A/C Heterodimerizer AP21967) Clontech Cat# 635055 DAPI (4’,6’-diamidine-2’-phenylindole dihydrochloride) Roche Cat# 10236276001 Tubulin TrackerTM Deep Red Thermo Fisher Scientific Cat# T34077 Mito Tracker RedTM Thermo Fisher Scientific Cat# M22425 Hoechst 33342 Cayman Cat# K9061 Dihydroethidium Cayman Cat# 12013 Percoll GE Healthcare Cat# 17-0891-01 Phusion High-Fidelity DNA-Polymerase New England Biolabs Cat# D22914 KAPA HiFi HotStart Roche Cat# KK2502 FirePol Solis BioDyne Cat# 01-01-02000 Biotin Sigma Aldrich Cat# B4639-100MG Streptavidin Sepharose GE Healthcare Cat# 17-5113-01 Triethylammonium bicarbonate buffer (TEAB) Sigma Aldrich Cat# T7408-500ML Tris(2-carboethyl)phosphine hydrochloride (TCEP) Sigma Aldrich Cat# C4706-2G Methyl methanethiosulfonate (MMTS) Thermo Fisher Scientific Cat# 23011 Iodoacetamide (IAA) Sigma Aldrich Cat# I1149-25G Trypsin/LysC Promega Cat# V5072 37% formaldehyde solution Sigma Aldrich Cat# 252549-25ML 20% formaldehyde-13C d2 solution Sigma Aldrich Cat# 596388-20G NaBH3CN Merck Cat# 818053 NaBD3CN Sigma Aldrich Cat# 190020-1G Critical commercial assays QIAGEN Plasmid Midi Kit Qiagen Cat# 12143 (Continued on next page) e1 Cell Systems 14, 9–23.e1–e7, January 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Monarch Genomic DNA Purification Kit NEB Cat# T3010S Deposited data Mass spectrometry data This study PRIDE: PXD035332 Experimental models: Cell lines Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-terminally fused to 2xFKBP-GFP-2xFKBP (C-termintegration cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) Wichers et al.22 N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-terminally fused to 2xFKBP-GFP2xFKBP (N-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-terminally fused to 2xFKBP-GFP2xFKBP (N-term-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-termintegration episomally expressing lyn-FRBmCherry or 1xNLS-FRB-mCherry (KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) episomally expressing 1xNLS-FRBmCherry (KS cell line) Wichers et al. 22 N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C3, ChrC13, Chr3C18 C-term-integration episomally expressing marker-mCherry (Colocalisation cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C7, ChrC16, SICs and NDC80 C-termintegration episomally expressing markermCherry and lyn-FRB (Colocalisation and KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-CL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C16 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Experimental models: Organisms/strains Plasmodium falciparum 3D7 Walliker et al.46 N/A (Continued on next page) Cell Systems 14, 9–23.e1–e7, January 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides For oligonucleotides used in this study see Table S2 this study N/A Recombinant DNA pSLI-sandwich Birnbaum et al.19 N/A pSLI- Chr3C1-11, Chr313-33, SIC1-5, NDC80-sandwich (see Table S2) this study N/A Chr3C12 pSLI-sandwich Wichers et al.22 N/A p-N-SLI-sandwich-K13-loxP Birnbaum et al.19 N/A p-N-SLI-Chr3C30-sandwich-loxP this study N/A p-N-SLI-Chr3C32-sandwich-loxP this study N/A 1xNLS-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A lyn-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A pSkipFlox Birnbaum et al.19 N/A pLyn-FRB-T2A-mCherry-yDHODHnmd3 Hoeijmakers et al.40 N/A pGRASP-mCherry-BSDnmd3 Birnbaum et al.20 N/A pARL-ama1ARO-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1RON12-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1AMA1-mCherry-yDHODH Wichers et al.22 N/A CEN3-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A NDC80-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A SKA-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A Chr3C16-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A BirA*-NL Birnbaum et al.20 N/A BirA*-CL Birnbaum et al.20 N/A Software and algorithms AxioVision release 4.7 Zeiss www.micro-shop.zeiss.com/en/ us/system/axiovision+softwaresoftware+axiovision-software/6014/ CorelDRAW X6 Corel Corporation www.coreldraw.com CorelPHOTOPAINT X6 Corel Corporation www.coreldraw.com FACSDiva version 6.1.3 BD Biosciences www.bdbiosciences.com/en-us/ products/software/instrumentsoftware/bd-facsdiva-software Graph Pad Prism 9 Graph Pad Software www.graphpad.com/scientific- software/prism/ ImageJ 2 open source47 https://imagej.nih.gov/ij/ ImageLab v5.2.1 Bio-Rad Laboratories www.bio-rad.com/de-de/ product/image-lab-software PyMOL Molecular Graphics System 2.5.2 Schrödinger, LLC https://pymol.org/2/ MaxQuant 1.5.3.30 open source from Cox lab (Cox et al.48) https://maxquant.org/ MaxQuant Perseus software package 1.4.0.20 open source from Cox lab (Tyanova et al.49) https://maxquant.org/perseus/ R i386 3.3.3 Open source https://www.r-project.org/

    Expressing:

    Article Title: Gene-by-gene screen of the unknown proteins encoded on Plasmodium falciparum chromosome 3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies monoclonal mouse anti-GFP Roche Cat# 11814460001; RRID: AB_390913 mouse anti-FKBP12 Santa Cruz Cat# SC-136962; RRID: AB_2102734 rabbit anti-Aldolase Mesén-Ramı́rez et al.45 N/A HRP conjugated goat anti-mouse Dianova Cat# DAB-87613 HRP conjugated anti-rabbit Dianova Cat# 111-035-045; RRID: AB_2337938 HRP conjugated Streptavidin Thermo Fisher Scientific Cat# SA10001; RRID: AB_2534069 Alexa FluorTM 594 conjugated Streptavidin Thermo Fisher Scientific Cat# S32356 Alexa FluorTM 488 conjugated goat anti-mouse Thermo Fisher Scientific Cat# A-11001 Bacterial and virus strains Escherichia coli XL-10 gold Tetr D(mcrA)183 D(mcrCBhsdSMRmrr) 173 endA1 supE44 thi-1recA1 gyrA96 relA1 lac Hte [F’ proABlacIqZ DM15 Tn10 (Tetr) Amy Camr] New England Biolabs Cat# C3040 Biological samples 0+ red blood cells (transfusion blood) University Medical Center Hamburg-Eppendorf (UKE) N/A Chemicals, peptides, and recombinant proteins WR99210 Jacobus Pharmaceuticals N/A Blasticidin S (BSD) Life Technologies Cat# R21001 DSM1 (MRA-1161) MRA/BEI resources Cat# MRA 1161 G418 Sigma Aldrich Cat# A1720 Rapalog (A/C Heterodimerizer AP21967) Clontech Cat# 635055 DAPI (4’,6’-diamidine-2’-phenylindole dihydrochloride) Roche Cat# 10236276001 Tubulin TrackerTM Deep Red Thermo Fisher Scientific Cat# T34077 Mito Tracker RedTM Thermo Fisher Scientific Cat# M22425 Hoechst 33342 Cayman Cat# K9061 Dihydroethidium Cayman Cat# 12013 Percoll GE Healthcare Cat# 17-0891-01 Phusion High-Fidelity DNA-Polymerase New England Biolabs Cat# D22914 KAPA HiFi HotStart Roche Cat# KK2502 FirePol Solis BioDyne Cat# 01-01-02000 Biotin Sigma Aldrich Cat# B4639-100MG Streptavidin Sepharose GE Healthcare Cat# 17-5113-01 Triethylammonium bicarbonate buffer (TEAB) Sigma Aldrich Cat# T7408-500ML Tris(2-carboethyl)phosphine hydrochloride (TCEP) Sigma Aldrich Cat# C4706-2G Methyl methanethiosulfonate (MMTS) Thermo Fisher Scientific Cat# 23011 Iodoacetamide (IAA) Sigma Aldrich Cat# I1149-25G Trypsin/LysC Promega Cat# V5072 37% formaldehyde solution Sigma Aldrich Cat# 252549-25ML 20% formaldehyde-13C d2 solution Sigma Aldrich Cat# 596388-20G NaBH3CN Merck Cat# 818053 NaBD3CN Sigma Aldrich Cat# 190020-1G Critical commercial assays QIAGEN Plasmid Midi Kit Qiagen Cat# 12143 (Continued on next page) e1 Cell Systems 14, 9–23.e1–e7, January 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Monarch Genomic DNA Purification Kit NEB Cat# T3010S Deposited data Mass spectrometry data This study PRIDE: PXD035332 Experimental models: Cell lines Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-terminally fused to 2xFKBP-GFP-2xFKBP (C-termintegration cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) Wichers et al.22 N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-terminally fused to 2xFKBP-GFP2xFKBP (N-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-terminally fused to 2xFKBP-GFP2xFKBP (N-term-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-termintegration episomally expressing lyn-FRBmCherry or 1xNLS-FRB-mCherry (KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) episomally expressing 1xNLS-FRBmCherry (KS cell line) Wichers et al. 22 N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C3, ChrC13, Chr3C18 C-term-integration episomally expressing marker-mCherry (Colocalisation cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C7, ChrC16, SICs and NDC80 C-termintegration episomally expressing markermCherry and lyn-FRB (Colocalisation and KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-CL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C16 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Experimental models: Organisms/strains Plasmodium falciparum 3D7 Walliker et al.46 N/A (Continued on next page) Cell Systems 14, 9–23.e1–e7, January 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides For oligonucleotides used in this study see Table S2 this study N/A Recombinant DNA pSLI-sandwich Birnbaum et al.19 N/A pSLI- Chr3C1-11, Chr313-33, SIC1-5, NDC80-sandwich (see Table S2) this study N/A Chr3C12 pSLI-sandwich Wichers et al.22 N/A p-N-SLI-sandwich-K13-loxP Birnbaum et al.19 N/A p-N-SLI-Chr3C30-sandwich-loxP this study N/A p-N-SLI-Chr3C32-sandwich-loxP this study N/A 1xNLS-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A lyn-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A pSkipFlox Birnbaum et al.19 N/A pLyn-FRB-T2A-mCherry-yDHODHnmd3 Hoeijmakers et al.40 N/A pGRASP-mCherry-BSDnmd3 Birnbaum et al.20 N/A pARL-ama1ARO-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1RON12-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1AMA1-mCherry-yDHODH Wichers et al.22 N/A CEN3-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A NDC80-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A SKA-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A Chr3C16-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A BirA*-NL Birnbaum et al.20 N/A BirA*-CL Birnbaum et al.20 N/A Software and algorithms AxioVision release 4.7 Zeiss www.micro-shop.zeiss.com/en/ us/system/axiovision+softwaresoftware+axiovision-software/6014/ CorelDRAW X6 Corel Corporation www.coreldraw.com CorelPHOTOPAINT X6 Corel Corporation www.coreldraw.com FACSDiva version 6.1.3 BD Biosciences www.bdbiosciences.com/en-us/ products/software/instrumentsoftware/bd-facsdiva-software Graph Pad Prism 9 Graph Pad Software www.graphpad.com/scientific- software/prism/ ImageJ 2 open source47 https://imagej.nih.gov/ij/ ImageLab v5.2.1 Bio-Rad Laboratories www.bio-rad.com/de-de/ product/image-lab-software PyMOL Molecular Graphics System 2.5.2 Schrödinger, LLC https://pymol.org/2/ MaxQuant 1.5.3.30 open source from Cox lab (Cox et al.48) https://maxquant.org/ MaxQuant Perseus software package 1.4.0.20 open source from Cox lab (Tyanova et al.49) https://maxquant.org/perseus/ R i386 3.3.3 Open source https://www.r-project.org/

    Marker:

    Article Title: Gene-by-gene screen of the unknown proteins encoded on Plasmodium falciparum chromosome 3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies monoclonal mouse anti-GFP Roche Cat# 11814460001; RRID: AB_390913 mouse anti-FKBP12 Santa Cruz Cat# SC-136962; RRID: AB_2102734 rabbit anti-Aldolase Mesén-Ramı́rez et al.45 N/A HRP conjugated goat anti-mouse Dianova Cat# DAB-87613 HRP conjugated anti-rabbit Dianova Cat# 111-035-045; RRID: AB_2337938 HRP conjugated Streptavidin Thermo Fisher Scientific Cat# SA10001; RRID: AB_2534069 Alexa FluorTM 594 conjugated Streptavidin Thermo Fisher Scientific Cat# S32356 Alexa FluorTM 488 conjugated goat anti-mouse Thermo Fisher Scientific Cat# A-11001 Bacterial and virus strains Escherichia coli XL-10 gold Tetr D(mcrA)183 D(mcrCBhsdSMRmrr) 173 endA1 supE44 thi-1recA1 gyrA96 relA1 lac Hte [F’ proABlacIqZ DM15 Tn10 (Tetr) Amy Camr] New England Biolabs Cat# C3040 Biological samples 0+ red blood cells (transfusion blood) University Medical Center Hamburg-Eppendorf (UKE) N/A Chemicals, peptides, and recombinant proteins WR99210 Jacobus Pharmaceuticals N/A Blasticidin S (BSD) Life Technologies Cat# R21001 DSM1 (MRA-1161) MRA/BEI resources Cat# MRA 1161 G418 Sigma Aldrich Cat# A1720 Rapalog (A/C Heterodimerizer AP21967) Clontech Cat# 635055 DAPI (4’,6’-diamidine-2’-phenylindole dihydrochloride) Roche Cat# 10236276001 Tubulin TrackerTM Deep Red Thermo Fisher Scientific Cat# T34077 Mito Tracker RedTM Thermo Fisher Scientific Cat# M22425 Hoechst 33342 Cayman Cat# K9061 Dihydroethidium Cayman Cat# 12013 Percoll GE Healthcare Cat# 17-0891-01 Phusion High-Fidelity DNA-Polymerase New England Biolabs Cat# D22914 KAPA HiFi HotStart Roche Cat# KK2502 FirePol Solis BioDyne Cat# 01-01-02000 Biotin Sigma Aldrich Cat# B4639-100MG Streptavidin Sepharose GE Healthcare Cat# 17-5113-01 Triethylammonium bicarbonate buffer (TEAB) Sigma Aldrich Cat# T7408-500ML Tris(2-carboethyl)phosphine hydrochloride (TCEP) Sigma Aldrich Cat# C4706-2G Methyl methanethiosulfonate (MMTS) Thermo Fisher Scientific Cat# 23011 Iodoacetamide (IAA) Sigma Aldrich Cat# I1149-25G Trypsin/LysC Promega Cat# V5072 37% formaldehyde solution Sigma Aldrich Cat# 252549-25ML 20% formaldehyde-13C d2 solution Sigma Aldrich Cat# 596388-20G NaBH3CN Merck Cat# 818053 NaBD3CN Sigma Aldrich Cat# 190020-1G Critical commercial assays QIAGEN Plasmid Midi Kit Qiagen Cat# 12143 (Continued on next page) e1 Cell Systems 14, 9–23.e1–e7, January 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Monarch Genomic DNA Purification Kit NEB Cat# T3010S Deposited data Mass spectrometry data This study PRIDE: PXD035332 Experimental models: Cell lines Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-terminally fused to 2xFKBP-GFP-2xFKBP (C-termintegration cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) Wichers et al.22 N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-terminally fused to 2xFKBP-GFP2xFKBP (N-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-terminally fused to 2xFKBP-GFP2xFKBP (N-term-integration cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3 candidates, SICs and NDC80 C-termintegration episomally expressing lyn-FRBmCherry or 1xNLS-FRB-mCherry (KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: PIC4-2xFKBP-GFP-2xFKBP (Chr3C12) episomally expressing 1xNLS-FRBmCherry (KS cell line) Wichers et al. 22 N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C3, ChrC13, Chr3C18 C-term-integration episomally expressing marker-mCherry (Colocalisation cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C30 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C32 N-term-integration episomally expressing diCre (cKO cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell lines: Chr3C7, ChrC16, SICs and NDC80 C-termintegration episomally expressing markermCherry and lyn-FRB (Colocalisation and KS cell lines; see Table S2) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C7 C-term-integration episomally expressing BirA*-CL-FRB-mCherry (DiQ-BioID cell line) this study N/A Plasmodium falciparum 3D7 knock-in cell line: Chr3C16 C-term-integration episomally expressing BirA*-NL-FRB-mCherry (DiQ-BioID cell line) this study N/A Experimental models: Organisms/strains Plasmodium falciparum 3D7 Walliker et al.46 N/A (Continued on next page) Cell Systems 14, 9–23.e1–e7, January 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides For oligonucleotides used in this study see Table S2 this study N/A Recombinant DNA pSLI-sandwich Birnbaum et al.19 N/A pSLI- Chr3C1-11, Chr313-33, SIC1-5, NDC80-sandwich (see Table S2) this study N/A Chr3C12 pSLI-sandwich Wichers et al.22 N/A p-N-SLI-sandwich-K13-loxP Birnbaum et al.19 N/A p-N-SLI-Chr3C30-sandwich-loxP this study N/A p-N-SLI-Chr3C32-sandwich-loxP this study N/A 1xNLS-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A lyn-FRB-mCherry-DHODHnmd3 Birnbaum et al.19 N/A pSkipFlox Birnbaum et al.19 N/A pLyn-FRB-T2A-mCherry-yDHODHnmd3 Hoeijmakers et al.40 N/A pGRASP-mCherry-BSDnmd3 Birnbaum et al.20 N/A pARL-ama1ARO-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1RON12-mCherry-yDHODH Wichers et al.22 N/A pARL-ama1AMA1-mCherry-yDHODH Wichers et al.22 N/A CEN3-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A NDC80-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A SKA-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A Chr3C16-mCherrysf3a2-lyn-FRB-yDHODHnmd3-T2A this study N/A BirA*-NL Birnbaum et al.20 N/A BirA*-CL Birnbaum et al.20 N/A Software and algorithms AxioVision release 4.7 Zeiss www.micro-shop.zeiss.com/en/ us/system/axiovision+softwaresoftware+axiovision-software/6014/ CorelDRAW X6 Corel Corporation www.coreldraw.com CorelPHOTOPAINT X6 Corel Corporation www.coreldraw.com FACSDiva version 6.1.3 BD Biosciences www.bdbiosciences.com/en-us/ products/software/instrumentsoftware/bd-facsdiva-software Graph Pad Prism 9 Graph Pad Software www.graphpad.com/scientific- software/prism/ ImageJ 2 open source47 https://imagej.nih.gov/ij/ ImageLab v5.2.1 Bio-Rad Laboratories www.bio-rad.com/de-de/ product/image-lab-software PyMOL Molecular Graphics System 2.5.2 Schrödinger, LLC https://pymol.org/2/ MaxQuant 1.5.3.30 open source from Cox lab (Cox et al.48) https://maxquant.org/ MaxQuant Perseus software package 1.4.0.20 open source from Cox lab (Tyanova et al.49) https://maxquant.org/perseus/ R i386 3.3.3 Open source https://www.r-project.org/



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